Motion Detection in Diffraction Tomography by Common Circle Methods

09/16/2022
by   Michael Quellmalz, et al.
0

The method of common lines is a well-established reconstruction technique in cryogenic electron microscopy (cryo-EM), which can be used to extract the relative orientations of an object in tomographic projection images from different directions. In this paper, we deal with an analogous problem in optical diffraction tomography. Based on the Fourier diffraction theorem, we show that rigid motions, i.e., a map composed of rotations and translations, can be determined by detecting common circles in the Fourier-transformed data. We introduce two methods based on the idea of identifying common circles to reconstruct the object motion: While the first one is motivated by the common line approach for projection images and detects the relative orientation by the shape of the common circles in the two images, the second one assumes a smooth motion over time and calculates the angular velocity of the rotational motion from an infinitesimal version of the common circle method. Interestingly, using the stereographic projection, both methods can be reformulated as common line methods, but these lines are, in contrast to those used in cryo-EM, not confined to pass through the origin and allow for a full reconstruction of the relative orientation. Numerical proof-of-the-concept examples demonstrate the performance of our reconstruction methods.

READ FULL TEXT

Please sign up or login with your details

Forgot password? Click here to reset